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Xavier_CarpFebruary 21st, 2008, 9:38 am
Posts: 13Hi guys, I have a question, does anyone collects bugs? if so how do you preserve aquatic insect larvae???

Thanks
KonchuFebruary 21st, 2008, 10:27 am
Site Editor
Indiana

Posts: 496
Here is some info on collecting and preservation of mayflies. These instructions will work for most other groups, too. Mayflies are delicate and need to be handled separately from other aquatic specimens. Other kinds of bugs have special handling, too. I'll sometimes carry some of the really soft things from the field alive and drop them into boiling water in a foam cup for a few seconds before transferring them to EtOH. A small, immersion heater will usually do the trick, plugged into an adapter that is plugged into the cig lighter of my vehicle.

http://mypage.iu.edu/~lmjacobu/collecting.html

"Collection fluid" usually refers to ethanol (EtOH). I recommend collecting into a solution of 90% EtOH/10% water. After an hour, or a little more, I'll drain off the solution and add more of the same concentration. I'll do this a few times until the liquid stops turning yellow. Finally, I'll put the specimens into a solution of 70% EtOH/30% water. However, if I am going to try to extract DNA from the specimens (ever), I'll collect into 100% (200 proof) EtOH and continue changes, using 100% each time. This often will leave specimens brittle, and sometimes they will extrude tissue. A final resting place of 70% is the best for medium-term storage and handling. Keeping specimens in alcohol in a freezer will extend their useful "life"time.

Best of luck.
Shawnny3February 21st, 2008, 8:03 pm
Moderator
Pleasant Gap, PA

Posts: 1197
I'll sometimes carry some of the really soft things from the field alive and drop them into boiling water in a foam cup for a few seconds before transferring them to EtOH.


You're a sick man, Konchu.

-Shawn
Jewelry-Quality Artistic Salmon Flies, by Shawn Davis
www.davisflydesigns.com
MartinlfFebruary 22nd, 2008, 2:23 pm
Moderator
Palmyra PA

Posts: 2968
Oh, and you can say this after digging some of those holes in the cornfield for me, Shawn?
"He spread them a yard and a half. 'And every one that got away is this big.'"

--Fred Chappell
Xavier_CarpFebruary 22nd, 2008, 5:44 pm
Posts: 13Konchu, thanks alot I'll do that, I really enjoy studying my bugs hehehe
Shawnny3February 23rd, 2008, 5:42 am
Moderator
Pleasant Gap, PA

Posts: 1197
Louis, that was completely different. Comparing insects with bait fishermen - apples and oranges.

-Shawn
Jewelry-Quality Artistic Salmon Flies, by Shawn Davis
www.davisflydesigns.com

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