Troutnut.com Fly Fishing for Trout Home
User Password
or register.
Scientific name search:

> > Bug collection

Xavier_CarpFebruary 21st, 2008, 9:38 am
Posts: 13Hi guys, I have a question, does anyone collects bugs? if so how do you preserve aquatic insect larvae???

Thanks
KonchuFebruary 21st, 2008, 10:27 am
Site Editor
Indiana

Posts: 496
Here is some info on collecting and preservation of mayflies. These instructions will work for most other groups, too. Mayflies are delicate and need to be handled separately from other aquatic specimens. Other kinds of bugs have special handling, too. I'll sometimes carry some of the really soft things from the field alive and drop them into boiling water in a foam cup for a few seconds before transferring them to EtOH. A small, immersion heater will usually do the trick, plugged into an adapter that is plugged into the cig lighter of my vehicle.

http://mypage.iu.edu/~lmjacobu/collecting.html

"Collection fluid" usually refers to ethanol (EtOH). I recommend collecting into a solution of 90% EtOH/10% water. After an hour, or a little more, I'll drain off the solution and add more of the same concentration. I'll do this a few times until the liquid stops turning yellow. Finally, I'll put the specimens into a solution of 70% EtOH/30% water. However, if I am going to try to extract DNA from the specimens (ever), I'll collect into 100% (200 proof) EtOH and continue changes, using 100% each time. This often will leave specimens brittle, and sometimes they will extrude tissue. A final resting place of 70% is the best for medium-term storage and handling. Keeping specimens in alcohol in a freezer will extend their useful "life"time.

Best of luck.
Shawnny3February 21st, 2008, 8:03 pm
Moderator
Pleasant Gap, PA

Posts: 1197
I'll sometimes carry some of the really soft things from the field alive and drop them into boiling water in a foam cup for a few seconds before transferring them to EtOH.


You're a sick man, Konchu.

-Shawn
Jewelry-Quality Artistic Salmon Flies, by Shawn Davis
www.davisflydesigns.com
MartinlfFebruary 22nd, 2008, 2:23 pm
Moderator
Palmyra PA

Posts: 2933
Oh, and you can say this after digging some of those holes in the cornfield for me, Shawn?
"He spread them a yard and a half. 'And every one that got away is this big.'"

--Fred Chappell
Xavier_CarpFebruary 22nd, 2008, 5:44 pm
Posts: 13Konchu, thanks alot I'll do that, I really enjoy studying my bugs hehehe
Shawnny3February 23rd, 2008, 5:42 am
Moderator
Pleasant Gap, PA

Posts: 1197
Louis, that was completely different. Comparing insects with bait fishermen - apples and oranges.

-Shawn
Jewelry-Quality Artistic Salmon Flies, by Shawn Davis
www.davisflydesigns.com

Quick Reply

You have to be logged in to post on the forum. It's this easy:
Username:          Email:

Password:    Confirm Password:

I am at least 13 years old and agree to the rules.

Related Discussions

TitleRepliesLast Reply
Re: Preservative solution % ratios ????
In the Photography Board by Brookyman
11Jun 22, 2012
by Brookyman
Re: Paraleptophlebia helena
(1 more)

In the Photography Board by Millcreek
13Aug 31, 2014
by Millcreek
Re: Air lock thingamabobbers
In Gear Talk by Adirman
6Apr 11, 2018
by Wygnar
Re: bug collection jars/vials?
In the Identify This! Board by Adirman
3May 19, 2011
by Adirman
Giant stonefly nymph
In the Photography Board by Jmd123
0
Re: Cleaning gear - Didymo
In General Discussion by Al514
3Jun 16, 2008
by Martinlf
Re: Easier way to get them out of their cases
In Brachycentrus Caddisfly Pupa by Dgracia
2Feb 15, 2013
by Troutnut
Re: fish id, weird brown?
In General Discussion by FishThirty
23Feb 10, 2012
by Czoom
Re: Finding Dobsonfly adults
In the Insect Family Corydalidae by Raider83
7Jul 3, 2008
by Shawnny3
More Baetis nymphs
(2 more)

In the Identify This! Board by Millcreek
0
Most Recent Posts
Re: Euro Nymph Rod
In Gear Talk by Wbranch (Barbaube replied)
Re: Floating line, WF or DT
In Gear Talk by Red_green_h (Wbranch replied)
Re: Long-time reader, never-time poster? Start here!
In General Discussion by Troutnut (Wbranch replied)
Re: Dubbing wax questions
In Fly Tying by Brian314 (Wbranch replied)
Re: Firehole Dry Fly hooks
In Fly Tying by Iasgair (TNEAL replied)
Invitation to the 33rd Slovenian Open Fly Tying Championship 2020
In General Discussion by Lucky_luke
Re: Fishing Rod Suggestions
In Gear Talk by Princemoga (Wbranch replied)
Re: Little Manistee
In Fishing Reports by Summer_doug
Re: Iowa Driftless
In General Discussion by KevinB (Red_green_h replied)
Re: Need an excuse to buy new rods
In Gear Talk by Barbaube (Red_green_h replied)